Oleate Prevents Palmitate-Induced Mitochondrial Dysfunction in Chondrocytes

The association between obesity and osteoarthritis (OA) in joints not subjected to mechanical overload, together with the relationship OA metabolic syndrome, suggests that there are systemic factors related disorders involved phenotype of OA. aim this work is study effects palmitate oleate on cellular metabolism an “ vitro ” model human chondrocytes. TC28a2 chondrocyte cell line was used analyze effect mitochondrial glycolytic function, Adenosine triphosphate (ATP) production lipid droplets accumulation. Palmitate, but oleate, produces dysfunction observed a lower coupling efficiency, maximal respiration spare respiratory capacity. Glycolytic function showed rates both capacity reserve when cells were incubated fatty acids (FAs). rate total ATP values chondrocytes palmitic acid (PA). formation increased FA conditions, being significantly higher oleic (OL). These results may help explain, at least part, close pathologies OA, as well elucidate some can define